Lethal Factor Active-Site Mutations Affect Catalytic Activity In Vitro
نویسندگان
چکیده
منابع مشابه
Two mutations in the dispensable part of alanine tRNA synthetase which affect the catalytic activity.
Two previously described chromosomal mutant alleles, alaS4 and alaS5, of Escherichia coli Ala-tRNA synthetase have been analyzed. Each causes a sharp diminution in aminoacylation activity and disrupts the alpha 4 tetramer structure of identical chains of 875 amino acids; neither mutation significantly disturbs the activity for synthesis of alanyladenylate. The location of each mutation within t...
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Glucose oxidase has wide applications in the pharmaceutical, chemical, and food industries. Many recent studies have enhanced key properties of this enzyme using directed evolution, yet without being able to reveal why these mutations are actually beneficial. This work presents a synergistic combination of experimental and computational methods, indicating how mutations, even when distant from ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1998
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.66.5.2374-2378.1998